Abstract Certolizumab pegol (CZP) is a Fab’ fragment of the humanized antibody with anti-TNF-α activity that is indicated as therapy for Crohn’s disease and rheumatoid arthritis. Using a BioSep-SEC-S3000 column (300 x 4.6 mm i.d., 5 µm particle size), a size exclusion liquid chromatography (SEC) method was developed. Mobile phase A consisted of 100 mM monobasic sodium phosphate and 200 mM sodium chloride (pH 7.0), while mobile phase B was ethanol (95:5, v/v), and the analysis was performed using a diode array detector (DAD) set to 214 nm and a flow rate of 0.5 ml min-1. In addition, a reversed-phase liquid chromatography (RP-LC) method based on gradient elution was developed on a Zorbax 300 SB C18 column (150 mm x 4.6 mm i.d., 3.5 µm particle size) kept at 80 °C. Mobile phase A was 0.1% (v/v) TFA in ultrapure water, and mobile phase B was a mixture of propanol, acetonitrile, ultrapure water and TFA (70 + 20 + 9.9 + 0.1, v/v) operated at a flow rate of 1.0 ml min-1, and DAD was applied at 214 nm. CZP elution was achieved with retention times of 5.6 min and 9.0 min for SEC and RP-LC, respectively.

RESUMO Objetivo descrever a elaboração de um objeto virtual de aprendizagem para o ensino da disciplina de história da enfermagem. Método descritivo, exploratório de relato de experiência sobre a construção do objeto de aprendizagem como metodologia ativa em aulas remotas da disciplina História da Enfermagem utilizando o modelo ADDIE, Analysis, Design, Development, Implementation e Evaluation no design instrucional. A disciplina ocorreu de dezembro de 2020 a março de 2021 em Macaé-RJ - Brasil. Resultados foram 30 horas, em 10 encontros síncronos e nove atividades assíncronas, participaram 26 estudantes pela plataforma MOODLE além de outras estratégias pedagógicas. Desafios foram principalmente de cunho motivacional, a interação discente com as plataformas demonstrou comprometimento e interesse, além destas terem sido fundamentais para o processo de ensino-aprendizagem ser dinâmico e prazeroso. Conclusão a construção de Objeto Virtual de Aprendizagem foi uma estratégia que auxiliou na melhora do rendimento discente além de promover uma inovação nos métodos pedagógicos docentes.

ABSTRACT Objective to describe the elaboration of a virtual learning object to teach the Nursing History academic discipline. Method a descriptive and exploratory study of the experience report type about the elaboration of a learning object as an active methodology in remote classes of the Nursing History academic discipline using the Analysis, Design, Development, Implementation and Evaluation (ADDIE) model of instructional design. The academic discipline was taught from December 2020 to March 2021 in Macaé, RJ, Brazil. Results the discipline had an hour load of 30 hours, distributed in 10 synchronous meetings and nine asynchronous activities, with the participation of 26 students through the MOODLE platform, in addition to other pedagogical strategies. The challenges were mainly of a motivational nature and the students’ interaction with the platforms showed commitment and interest; moreover, they were fundamental to turning learning-teaching into a dynamic and pleasant process. Conclusion elaboration of the Virtual Learning Object was a strategy that assisted in improving the students’ performance, in addition to promoting innovation in the teachers’ pedagogical methods.

RESUMEN Objetivo describir la elaboración de un objeto virtual de aprendizaje para enseñar la asignatura académica de Historia de la Enfermería. Método informe de experiencia descriptivo y exploratorio sobre la elaboración del objeto de aprendizaje como metodología activa en clases a distancia de la asignatura Historia de la Enfermería utilizando el modelo ADDIE (Análisis, Diseño, Desarrollo, Implementación y Evaluación) en el diseño instruccional. La asignatura se dictó entre diciembre de 2020 y marzo de 2021 en Macaé, RJ, Brasil. Resultados tuvo una carga horaria de 30 horas, distribuidas en 10 encuentros sincrónicos y nueve actividades asincrónicas, con la participación de 26 estudiantes a través de la plataforma MOODLE, además de otras estrategias pedagógicas. Los desafíos fueron principalmente de carácter motivacional y la interacción de los estudiantes con las plataformas demostró compromiso e interés, además de ser fundamental para que el proceso de enseñanza-aprendizaje sea dinámico y ameno. Conclusión elaborar el Objeto Virtual de Aprendizaje representó una estrategia que contribuyó a mejorar el rendimiento de los estudiantes, además de promover la innovación en los métodos pedagógicos de los profesores.

Recombinant human interferon beta 1b (rhIFNβ-1b) is clinically used to treat multiple sclerosis. A reversed-phase liquid chromatography (RP-LC) method was carried out on a Jupiter C4 column (250 mm × 4.6 mm i.d.). The mobile phase A consisted of 0.1% trifluoroacetic acid (TFA) in water, and the mobile phase B was acetonitrile with 0.1% TFA run at a flow rate of 1.0 mL/min. A size exclusion liquid chromatography (SE-LC) method was carried out on a BioSep-SEC-S 2000 column (300 mm × 7.8 mm i.d.). The mobile phase consisted of 1 mM monobasic potassium phosphate, 8 mM sodium phosphate dibasic and 200 mM sodium chloride buffer pH 7.4, run isocratically at a flow rate of 0.8 mL/min. Retention times were 31.87 and 17.78 min, and calibration curves were linear over the concentration range of 1-200 µg/mL (r2 = 0.9998) and 0.50-200 µg/mL (r2 = 0.9999), respectively, for RP-LC and SE-LC, with detection at 214 nm. Liquid chromatography (LC) methods were validated and employed in conjunction with the in vitro bioassay to assess the content/potency of rhIFNβ-1b, contributing to improve the quality control and to ensure the efficacy of the biotherapeutic.

Resumo: Nos últimos anos houve um aumento no interesse em pesquisas em remanescentes de Floresta Atlântica acima de 500 m de altitude, como na Serra da Mantiqueira, a qual é considerada área prioritária para a conservação. A fauna de quirópteros na Serra da Mantiqueira ainda permanece relativamente pouco estudada, e aqui nós apresentamos uma lista de espécies de morcegos da Reserva Particular do Patrimônio Natural (RPPN) Cachoeira do Tombo, uma unidade de conservação privada localizada em área de Floresta Atlântica Montana na Serra da Mantiqueira. Os morcegos foram capturados com redes de neblina ao longo de trilhas e junto de um abrigo diurno. Um total de 498 indivíduos de 19 espécies de morcegos pertencentes às famílias Phyllostomidae, Vespertilionidae e Molossidae foram capturados. Phyllostomidae foram capturados somente em trilhas, Molossidae no abrigo diurno e Vespertilionidae em ambos. As curvas de acumulação de espécies não mostraram tendência de estabilização. No entanto, 80% da riqueza esperada foi amostrada e a riqueza de espécies encontrada foi similar a outros estudos previamente conduzidos na região. Diferente de outros inventários realizados na Mata Atlântica, Desmodus rotundus foi a espécie mais frequentemente capturada ao longo das trilhas. Um grande número de indivíduos de Molossus aztecus foi encontrado se abrigando em abrigo artificial, o que constitui um evento pouco usual. Adicionalmente, a coabitação dessa espécie com Molossus molossus é reportada aqui pela primeira vez. Os resultados mostram que essa área, que está inserida no mapa de conflitos ambientais do estado de Minas Gerais, possui uma rica chiropterofauna e corrobora a importância de usar redes de neblina em abrigos para aumentar a probabilidade de capturar a riqueza e abundância de morcegos insetívoros presentes na área.

Abstract: In recent years there has been an increase in research interest in remnants of Atlantic Forest above 500 m a.s.l., such as in the Serra da Mantiqueira, which is considered a priority area for conservation. The chiropterofauna of the Serra da Mantiqueira remains relatively under-studied, and here we present a list of bat species from the "Reserva Particular do Patrimônio Natural (RPPN) Cachoeira do Tombo", a private conservation unit located in an area of montane Atlantic Forest in the Serra da Mantiqueira. Bats were captured with mist nets along trails and near a diurnal roost. A total of 498 individuals of 19 bat species belonging to the families Phyllostomidae, Vespertilionidae and Molossidae were captured. Phyllostomidae were captured only along the trails, Molossidae were captured only in the diurnal roost and Vespertilionidae were captured in both. The species accumulation curves did not show stabilizing trends. However, 80% of the expected richness was sampled and the species richness of bats found is similar to other studies previously carried out in the region. In contrast to other inventories carried out in the Atlantic Forest, Desmodus rotundus was the species most frequently captured along the trails. A large number of individuals of Molossus aztecus sheltering in man-made structures were caught, constituting an unusual event. Additionally, here we report cohabitation of this species with Molossus molossus for the first time. Our results show that this area, which appears on the map of environmental conflicts for the state of Minas Gerais, has a rich chiropterofauna and also further corroborate the importance of using mist-nets at roosts to increase the probability of capturing the richness and abundance of insectivorous bats present in the area.

Myotis izecksohni Moratelli et al., 2011 foi recentemente descrita a partir da revisão do complexo Myotis nigricans (Schinz, 1821), com base em amostras de quatro localidades acima de 700 m de altitude na Mata Atlântica do Sul (Paraná) e sudeste (Rio de Janeiro) do Brasil. A espécie está atualmente registrada apenas nessas localidades e sua ecologia, história natural e limites de distribuição são pouco conhecidos. Aqui, são apresentados 3 novos registros de ocorrência para o estado de Minas Gerais, sudeste do Brasil. Os caracteres qualitativos e quantitativos dos espécimes aqui examinados enquadram-se naqueles da descrição original de M. izecksohni.

Myotis izecksohni Moratelli et al., 2011 was recently described from the Myotis nigricans (Schinz, 1821) complex, based on samples from four localities above 700 m in the Brazilian Atlantic Forest of south (Paraná) and southeastern (Rio de Janeiro) Brazil. The species is currently known only from these localities and little is known about its distributional limits, ecology and natural history. Here, we present 3 new occurrence records from Minas Gerais, southeastern Brazil. Specimens reported here fit the qualitative and quantitative traits reported in the original description of M. izecksohni.